Estimation of alkaline phosphatase in serum – liver function tests


Estimation of alkaline phosphatase in serum Introduction Phosphatase are enzymes which catalyze the splitting of phosphoric acid from certain mono phosphoric esters such as (glycerophosphates and phenyl phosphates) Alkaline phosphatase with a maximum activity at about PH 10 ↚ Principle Alkaline phosphatase in serum acts on the substrate disodium phenyl phosphate  buffered at PH 10 at 37ᵒ to liberate phenol This phenol condenses with 4- amino antipyrine in the presence of alkaline oxidizing agent potassium ferricyanide to give red colored complex which is measured at 520 nm Normal range                 …

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