Estimation of Albumin in serum – liver function tests


Estimation of Albumin in serum Introduction ·       Albumin accounts 60% of human plasma protein ·       It binds to water-insoluble substances (as bilirubin, fatty acids , or hormones) ·       Regulate the osmotic pressure of blood ·       Albumin is synthesized by liver using dietary proteins Principle ·       Under acidic conditions , Albumin present in serum sample binds to bromo cresol green to give a blue – green colored complex which read at 630 nm ·       The intensity of color is directly proportional to Albumin concentration Albumin + bromo cresol green…

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