Estimation of Albumin in serum - liver function tests | ALM3ML

Estimation of Albumin in serum

Introduction

· Albumin accounts 60% of human plasma protein

· It binds to water-insoluble substances (as bilirubin, fatty acids , or hormones)

· Regulate the osmotic pressure of blood

· Albumin is synthesized by liver using dietary proteins

Principle

· Under acidic conditions , Albumin present in serum sample binds to bromo cresol green to give a blue – green colored complex which read at 630 nm

· The intensity of color is directly proportional to Albumin concentration

Albumin + bromo cresol green → Albumin – bromo cresol green complex (blue green color)

Normal range

· Albumin : 3.5 – 5.5 g %

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Reagents

· Citrate buffer : give acidic medium

· Bromo cresol green : color reagent

· Bovine serum albumin (BSA) : standard protein

· Detergent : it prevent the conversion of bromo cresol green from green to dark blue , Buffer isn’t completely soluble ,but with detergent it becomes completely soluble

Procedure

Depend on colorimetric technique

Reagent	Test	Standard	Blank
Serum	20µ	–	–
St-protein	–	20µ	–
Color reagent	3ml	3ml	3ml
	Mix well ,stand for 5 min at RT , then read at 630 nm

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Calculations

Serum Albumin level increased in:

Dehydration
Poor protein utilization

Serum Albumin level decreased in:

· Malnutrition

· Liver dysfunction (Ascites)

Procedure with kits in lab :

Depend on colorimetric technique
1000µ Albumin reagent + 10µ serum , Mix well, stand for 5 min at RT then read

Estimation of Albumin in serum – liver function tests